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pericyte growth medium pgm  (PromoCell)


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    PromoCell pericyte growth medium pgm
    Pericyte Growth Medium Pgm, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pericyte growth medium pgm/product/PromoCell
    Average 95 stars, based on 90 article reviews
    pericyte growth medium pgm - by Bioz Stars, 2026-03
    95/100 stars

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    a, Schematic of microwell plates in 96-well plate format and representative phase contrast image of a GRi3D organoid array within a single well. Scale bar, 1 mm. b, Quantification of blood-brain barrier organoid diameter in GRi3D microwell arrays within 24- or 96-well plate compared to organoids grown on agarose substrate. Each dot represents a single organoid, while different colors represent independent experiments. c, Representative confocal images of a blood-brain barrier organoid labelled with cell type-specific markers showing specific layering and organization of brain endothelial cells (P-gp, magenta), <t>pericytes</t> (NG2, green) and astrocytes (GFAP, cyan) when grown in GRi3D arrays. Nuclei are labelled with DAPI (grey). Scale bars, 100 μm. d, Representative fluorescent image of a live/dead staining showing the viability of blood-brain barrier organoids grown on GRi3D arrays. Calcein AM in green labels live cells whereas ethidium homodimer-1 in magenta labels dead cells. Scale bar, 1 mm. Images on the right show a higher magnification of the boxed area. Scale bar 500 μm.
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    a, Schematic of microwell plates in 96-well plate format and representative phase contrast image of a GRi3D organoid array within a single well. Scale bar, 1 mm. b, Quantification of blood-brain barrier organoid diameter in GRi3D microwell arrays within 24- or 96-well plate compared to organoids grown on agarose substrate. Each dot represents a single organoid, while different colors represent independent experiments. c, Representative confocal images of a blood-brain barrier organoid labelled with cell type-specific markers showing specific layering and organization of brain endothelial cells (P-gp, magenta), pericytes (NG2, green) and astrocytes (GFAP, cyan) when grown in GRi3D arrays. Nuclei are labelled with DAPI (grey). Scale bars, 100 μm. d, Representative fluorescent image of a live/dead staining showing the viability of blood-brain barrier organoids grown on GRi3D arrays. Calcein AM in green labels live cells whereas ethidium homodimer-1 in magenta labels dead cells. Scale bar, 1 mm. Images on the right show a higher magnification of the boxed area. Scale bar 500 μm.

    Journal: bioRxiv

    Article Title: Investigating receptor-mediated antibody transcytosis using Blood-Brain Barrier organoid arrays

    doi: 10.1101/2021.02.09.430382

    Figure Lengend Snippet: a, Schematic of microwell plates in 96-well plate format and representative phase contrast image of a GRi3D organoid array within a single well. Scale bar, 1 mm. b, Quantification of blood-brain barrier organoid diameter in GRi3D microwell arrays within 24- or 96-well plate compared to organoids grown on agarose substrate. Each dot represents a single organoid, while different colors represent independent experiments. c, Representative confocal images of a blood-brain barrier organoid labelled with cell type-specific markers showing specific layering and organization of brain endothelial cells (P-gp, magenta), pericytes (NG2, green) and astrocytes (GFAP, cyan) when grown in GRi3D arrays. Nuclei are labelled with DAPI (grey). Scale bars, 100 μm. d, Representative fluorescent image of a live/dead staining showing the viability of blood-brain barrier organoids grown on GRi3D arrays. Calcein AM in green labels live cells whereas ethidium homodimer-1 in magenta labels dead cells. Scale bar, 1 mm. Images on the right show a higher magnification of the boxed area. Scale bar 500 μm.

    Article Snippet: Human brain microvascular pericytes (HBVP, ScienCell Research Laboratories) were cultured in Pericyte Growth Medium (PGM) composed of Pericytes Medium (PM, ScienCell Research Laboratories) supplemented with 2% FBS, 1% Pericytes Growth Supplement (PGS, ScienCell Research Laboratories) and 1% penicillin/streptomycin.

    Techniques: Staining